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重组人G-CSF
Recombinant Human G-CSF
产品编号: HZ-1208
规  格:
单  价: 询价
生 产 商: Humanzyme
到货时间: 3-4weeks
购买数量:
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Product Description:

 

  Human Cell Expressed
  Animal-derived product free
  Endotoxin-free*
  Available in Bulk
  High Potency for Hematopoietic Cell Growth
  Authentic Glycosylation

HumanKine® G-CSF is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 21 to 25 kDa. This molecular mass is due to glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications. This cytokine is produced in a serum-free, chemically defined media. The purity is greater than 95%.

Technical Specifications:

 

  Species: Human
  Expression: Human Cell Expressed
  Activity: 0.02 to 0.08 ng/mL ED50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 21 to 25 kDa, monomer, glycosylated.

Activity Assay:

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line).

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

[1]Smith-Berdan S, Nguyen A, Hassanein D, Zimmer M, Ugarte F, Ciriza J, Li D, Garci´a-Ojeda ME, Hinck L, Forsberg EC. 2011. Robo4 Cooperates with Cxcr4 to Specify Hematopoietic Stem Cell Localization to Bone Marrow Niches. Cell Stem Cell. 8: 72. [PMID: 21211783]

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.