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重组人FLT3 Ligand
Recombinant Human FLT3 Ligand
产品编号: HZ-1150
规  格:
单  价: 询价
生 产 商: Humanzyme
到货时间: in store
购买数量:
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Product Description:

 

  Human Cell Expressed
  Animal-derived product free
  Endotoxin-free*
  Available in Bulk
  High Activity
  Authentic Glycosylation

HumanKine® FLT3 Ligand is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 24 to 30 kDa. This cytokine is produced in a serum-free, chemically defined media. The molecular mass is a narrower range than reported when this cytokine is expressed in insect Sf21 cells (17 to 30 kDa), emphasizing the difference in glycosylation in different expression systems.

Technical Specifications:

 

  Species: Human
  Expression: Human Cell Expressed
  Activity: 0.2 to 0.8 ng/mL ED50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 24 to 30 kDa, monomer, glycosylated

Activity Assay:

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

[1]Byrnes C, Lee YT, Meier ER, Rabel A, Sacks DB, Miller JL. 2013. Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium. J Tissue Eng Regen Med [PMID:23606586]
[2]Woods N, Parker AS, Moraghebi R, Lutz MK, Firth AL, Brennand KJ, Berggren WT, Raya A, Belmonte JCI, Gage FH, Verma IM. 2011. Efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines. Stem Cells. 29: 1158. [PMID: 21544903]

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.