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重组人Cystatin C
Recombinant Human Cystatin C
产品编号: HZ-1210
规  格:
单  价: 询价
生 产 商: Humanzyme
到货时间: in store
购买数量:
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Product Description:

  Human Cell Expressed
  Animal-derived product free
  Endotoxin-free*
  Available in Bulk
  High Activity

HumanKine® Cystatin C is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cystatin C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.

 

Technical Specifications:

  Species: Human
  Expression: Human Cell Expressed
  Activity: 0.3 to 1.5 µM IC50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 12 to 13 kDa, monomer, non-glycosylated

Activity Assay:

The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 µg/mL (0.3 to 1.5 µM) with a range of 1.56µg/mL to 50µg/mL cystatin C in presence of 0.55µM papain and 0.44µM L-BAPA.

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.