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重组人BMP-2
Recombinant Human BMP-2
产品编号: HZ-1126
规  格:
单  价: 询价
生 产 商: Humanzyme
到货时间: in store
购买数量:
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Product Description:

 

  Human Cell Expressed
  Animal-derived product free
  Endotoxin-free*
  Available in Bulk
  High Activity
  Glycosylated disulfide–linked homodimer

HumanKine® BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 30 to 38 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. The purity is greater than 95%.

 

Technical Specifications:

 

  Species: Human
  Expression: Human Cell Expressed
  Activity: 20 to 100 ng/mL ED50
  Purity: >95%
  Endotoxin: Endotoxin-free*
  Molecular Mass: 30 to 38 kDa, homodimer, glycosylated

Activity Assay:

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (Mouse chondrogenic cell line).

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.


*Endotoxin-free: <0.5 EU/µg

All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.

[1]Ookuma YF, Kiyoshima T, Kobayashi I, Nagata K, Wada H, Fujiwara H, Yamaza H, Nonaka K, Sakai H. 2012. Multiple functional involvement of Thymosin beta-4 in tooth germ development. Histochemistry and Cell Biology. [PMID: 23052839]
[2]Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness Jr. CT, West MD. 2012. A human embryonic stem cell-derived clonal progenitor cell line with chodrogenic potential and markers of craniofacial mesenchyme. Regenerative Medicine. [PMID: 22519755]
[3]Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S, Yamada Y. 2011. Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation. The Journal of Cellular Biology. 193: 1257. [PMID: 21690309] Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y. 2011. Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation. The Journal of Biological Chemistry. 265: 18948. [PMID: 20404334]

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Stem cell reserch
HumanZyme develops and manufactures growth factors and cytokines for stem cell research applications. HumanKine® cytokines and growth factors are applied to stem cells for culture expansion or to drive differentiation into specific cell types. The human cell manufacturing system ensures native human glycosylation and other human-specific modifications that can result in better bioactivity and enhanced half-life.

Immunology Research
HumanZyme develops and manufactures cytokines and cell culture media for immunology applications. Cytokines are cell signaling molecules that enable cell-cell communication, stimulation, differentiation, and other processes. HumanKine® cytokines, expressed in human cells, possess authentic human glycosylation, folding, disulfide bonds, and other modifications which are important for biological activity, half-life, and human-specific functions.

Human Cellular Therapy
HumanKine® cytokines and growth factors originally developed for stem cell applications are beginning to be utilized by cellular therapy developers in early stage trials. Growth factors and cytokines are often employed ex vivo to drive stem cell differentiation into potentially therapeutic cells. For many growth factors and cytokines, the industry lacks cGMP proteins needed for late stage clinical trials and subsequent regulatory approval of promising cellular therapies. HumanKine proteins can be adapted to cGMP production.