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T4 DNA Ligase
T4 DNA Ligase
产品编号: MB100-0100
规  格:
单  价: 询价
生 产 商: Genedirex
到货时间: 3-4weeks
购买数量:
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Description

The T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-OH termini and 5′-P termini. Its MW is
about 62,000, and its optimal reaction pH is 7.6. It requires Mg2+ and ATP as cofactors. This enzyme can ligate both
cohesive end pair and blunt end pair.
Unit DefinitionOne unit is the amount of enzyme that ligates more than 90% of 6 μg of λDNA-HindIII fragments in a 20 μl mixture in 30
minutes at 16°C.
One unit of this enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in 66 mM Tris-HCl (pH 7.6) that
contains 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, and 3.3 μM [32P]-Na4P2O7.

 

Size: 100 units

产品编号 产品名称 规格 单价 生产商 到货时间 说明书
DM001-R500100bp DNA Ladder RTU (Ready-to-Use)500ul800元Genedirex3-4weeks
DM003-R500100bp DNA Ladder H3 RTU (Ready-to-Use)500ul800元Genedirex3-4weeks
DM010-R5001Kb DNA Ladder RTU (Ready-to-Use)500ul800元Genedirex3-4weeks
DM011-R500Kplus DNA Ladder RTU (Ready-to-Use)500ul1280元Genedirex3-4weeks
DM013-R500XLarge DNA Ladder RTU (Ready-to-Use)500ul1280元Genedirex3-4weeks
DM012-R50050bp DNA Ladder RTU (Ready-to-Use)500ul1280元Genedirex3-4weeks
LD001-1000Novel Juice (DNA Staining Reagent)1ml450元Genedirex3-4weeks
DN001010 mM dNTP Mix1ml400元Genedirex3-4weeks

PCR